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Recombinant Human IgG1 Fc Protein (Biotin)

Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330 (Asp239Glu, Leu241Met) is expressed with a Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

G gamma-1 chain C region; IGHG1

UniProt

P01857

Expression Region

Asp104-Lys330 (Asp239Glu, Leu241Met)

Conjugation

Biotin

Tag

C-Avi

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Biotinylated Human IgG1 Fc (Cat#orb2993718) at 2μg/ml (100 μl/well) can bind Recombinant Protein G (Cat#orb2993145) . The ED50 of Recombinant Protein G (Cat#orb2993145) is 49.41 ng/ml. (Regularly tested) . BLI: Loaded Biotinylated Human IgG1 Fc-His-Avi (Cat#orb2993718) on SA Biosensor, can bind Protein G-Fc (Cat#orb2993145) with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human IgG1 Fc (Cat#orb2993718) on SA Biosensor, can bind Recombinant Human CD64 (C-6His) (Cat#orb2994349) with an affinity constant of 1.23 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 27.7 KDa. Observed: 33 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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