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Dvl1 (mouse) Antibody

Goat polyclonal to Dvl1

Product Specifications

Product Name Alternative

Dvl1; dishevelled, dsh homolog 1 (Drosophila) ; RP23-242K15.15-003; Dvl; mKIAA4029; DSH homolog 1; dishevelled-1; segment polarity protein dishevelled homolog DVL-1

Reactivity

Human, Mouse

Target

Dvl1 (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

PQLEEAPLTVKSD

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

1-3 μg/ml

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

75.4; 63.0

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 80kDa band observed in lysates of cell line NIH3T3 (calculated MW of 75.4kDa according to NP_034221.3) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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