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Recombinant Human DLL4 Protein

Recombinant Human Delta-like Protein 4 is produced by our Mammalian expression system and the target gene encoding Ser27-Pro524 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Delta-like protein 4; Drosophila Delta homolog 4; Delta4; DLL4

UniProt

Q9NR61

Expression Region

Ser27-Pro524

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human DLL4 Antibody (Demcizumab, Research Grade) at 1μg/ml (100 μl/well) can bind Recombinant Human DLL4 (C-Fc) (Cat#orb2993713) *. The EC50 of Recombinant Human DLL4 (C-Fc) (Cat#orb2993713) is 3.48 ng/ml (QC verified) .*: Biotinylated by NHS-biotin prior to testing.

Form

Lyophilized

Molecular Weight

Predicted: 81.2 KDa. Observed: 85-100 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH 8.5.

Available Sizes

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