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Recombinant Human Frizzled-8 Protein (Biotin)

Biotinylated Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His, Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

Frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8

UniProt

Q9H461

Expression Region

Ala28-Pro172

Conjugation

Biotin

Tag

C-6His-Avi

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Biotinylated Human Frizzled-8 (C-6His-Avi) (Cat#orb2993701) at 2 μg/ml (100 μl/well) can bind Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) . The ED50 of Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) is 9.67 ng/ml. (Regularly tested) .. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on Pro-A Biosensor, can bind Biotinylated Human Frizzled-8 (C-6His-Avi) (Cat#orb2993701) with an affinity constant of 0.88 μM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 19.1 KDa. Observed: 25-35 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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