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Recombinant Human TGF-beta 3 Protein (Biotin)

Biotinylated Recombinant Human Transforming Growth Factor Beta-3 Proprotein is produced by our Mammalian expression system and the target gene encoding Ala301-­Ser412 (Tyr340Phe) is expressed with a Avi tag at the N-terminus.

Product Specifications

Product Name Alternative

TGFB3; ARVD; TGF-beta3

UniProt

P10600

Expression Region

Ala301-­Ser412 (Tyr340Phe)

Tag

N-Avi

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human TGFBR2-Fc (Cat#orb3002421) at 2μg/ml (100 μl/well) can bind Biotinylated Human TGF-beta 3-Avi (Cat#orb2993088) . The ED50 of Biotinylated Human TGF-beta 3-Avi (Cat#orb2993088) is 2.05 ng/ml.. Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‐1 human erythroleukemic cells.The ED50 for this effect is 0.37ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 14.5 KDa. Observed: 10-14 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 4mM HCl.

Available Sizes

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