What is the catalog number for Recombinant Human RSPO3 Protein?

The catalog number for Recombinant Human RSPO3 Protein is orb2993001.

What sizes are available for Recombinant Human RSPO3 Protein?

Recombinant Human RSPO3 Protein is available in the following size(s): 10 µg, 50 µg, 500 µg, 1 mg.

How many pack sizes does Recombinant Human RSPO3 Protein come in?

Recombinant Human RSPO3 Protein is available in 4 different pack sizes.

Is Recombinant Human RSPO3 Protein in stock?

Recombinant Human RSPO3 Protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Human RSPO3 Protein

Recombinant Human R-Spondin 3 is produced by our Mammalian expression system and the target gene encoding Gln22-Val146 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

R-spondin-3; RSPO3; Protein with TSP type-1 repeat; Roof plate-specific spondin-3; Thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1

UniProt

Q9BXY4

Expression Region

Gln22-Val146

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) . SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)

Bioactivity

Cell Based Assay: Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494), R-spondin 3 (Cat#orb2993001), KGF (Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753) . DAPI (blue), Ki67 (red) and ER (green) .

Form

Lyophilized

Molecular Weight

Predicted: 14.7 kDa. Observed: 15-25 kDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.