Recombinant Human Siglec-3 Protein
Recombinant Human Myeloid cell surface antigen CD33 is produced by our Mammalian expression system and the target gene encoding Asp18-His259 is expressed with a mouse IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Myeloid Cell Surface Antigen CD33; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67; CD33; SIGLEC3
Expression Region
Asp18-His259
Conjugation
Unconjugated
Tag
C-mFc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human Siglec-3 (C-mFc) (Cat#orb2992990) at 2μg/ml (100 μl/well) can bind Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) *. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) is 7.679 ng/ml. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 53.5 kDa. Observed: 65-90 kDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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