Recombinant Cynomolgus TSLP Protein (Biotin)
Biotinylated Recombinant Cynomolgus Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 is expressed with a 6His, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; TSLP
Expression Region
Tyr29-Gln159
Conjugation
Unconjugated
Tag
C-6His-Avi
Origin
Cynomolgus
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) . SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
ELISA: Immobilized Anti-Human TSLP mAb (Cat#orb2992721) at 1μg/ml (100 μl/well) can bind Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) .The ED50 of Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) is 0.097 ng/ml. (Regularly tested) . BLI: Loaded Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) on SA Biosensor, can bind Human TSLPR&IL-7RA Heterodimer-His (Cat#orb2993098) with an affinity constant of 2.15 nM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Cynomolgus TSLP-His-Avi (Cat#orb2992989) on SA Biosensor, can bind Anti-Human TSLP mAb (Cat#orb2992721) with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 17.8 kDa. Observed: (10-13) & (16-29) KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.
Available Sizes
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