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Recombinant Human TSLP Protein (Biotin)

Biotinylated Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159 is expressed with a 10His, Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; TSLP

UniProt

Q969D9

Expression Region

Tyr29-Gln159

Conjugation

Unconjugated

Tag

C-10His-Avi

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) . SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)

Bioactivity

ELISA: Immobilized Anti-Human TSLP mAb-Fc (Cat#orb2992721) at 1μg/ml (100 μl/well) can bind Biotinylated Human TSLP-His-Avi (Cat#orb2992987) .The ED50 of Biotinylated Human TSLP-His-Avi (Cat#orb2992987) is 0.851 ng/ml. (Regularly tested) . BLI: Loaded Biotinylated Human TSLP-His-Avi (Cat#orb2992987) on SA Biosensor, can bind Anti-Human TSLP mAb (Cat#orb2992721) with an affinity constant of 46.9 pM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 18.1 kDa. Observed: (10-13) & (16-29) kDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4

Available Sizes

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