Nectin-4 Human Recombinant Protein (Biotin)
Recombinant Human Nectin-4 is produced by our Mammalian expression system and the target gene encoding Gly32-Leu153 is expressed with a 6His, Avi tag at C-terminus.
Product Specifications
Product Name Alternative
PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
UniProt
Q96NY8
Expression Region
Gly32-Leu153
Conjugation
Unconjugated
Tag
C-6His-Avi
Origin
Human
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Anti-Human Nectin-4 mAb (Cat# orb2993141) at 2μg/ml (100 μl/well) can bind Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) . The ED50 of Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) is 0.035 ng/ml. (Regularly tested) . BLI: Loaded Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) on SA Biosensor, can bind Anti-Human Nectin-4 mAb (Cat#orb2993141) with an affinity constant of 3.74 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 16 KDa. Observed: 15-21 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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