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Nectin-4 Human Recombinant Protein (Biotin)

Recombinant Human Nectin-4 is produced by our Mammalian expression system and the target gene encoding Gly32-Leu153 is expressed with a 6His, Avi tag at C-terminus.

Product Specifications

Product Name Alternative

PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR

UniProt

Q96NY8

Expression Region

Gly32-Leu153

Conjugation

Unconjugated

Tag

C-6His-Avi

Origin

Human

Field of Research

Cancer Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Anti-Human Nectin-4 mAb (Cat# orb2993141) at 2μg/ml (100 μl/well) can bind Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) . The ED50 of Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) is 0.035 ng/ml. (Regularly tested) . BLI: Loaded Biotinylated Human Nectin-4 (C-6His-Avi) (Cat#orb2992962) on SA Biosensor, can bind Anti-Human Nectin-4 mAb (Cat#orb2993141) with an affinity constant of 3.74 pM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 16 KDa. Observed: 15-21 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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