Recombinant Human B7-H3 Protein (Biotin)
Biotinylated Recombinant Human CD276 antigen is produced by our Mammalian expression system and the target gene encoding Leu29-Thr461 is expressed with a 6His, Avi tag at C-terminus.
Product Specifications
Product Name Alternative
CD276; B7H3; 4Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule
UniProt
Q5ZPR3
Expression Region
Leu29-Thr461
Conjugation
Unconjugated
Tag
C-6His-Avi
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Biotinylated Human B7-H3 (C-6His-Avi) (Cat#orb2992954) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H3 mAb-Fc (Cat#orb2993104) . The ED50 of Anti-Human B7-H3 mAb-Fc (Cat#orb2993104) is 11.42 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human B7-H3 mAb (Cat#orb2993104) on Pro-A Biosensor, can bind Biotinylated Human B7-H3 (C-6His-Avi) (Cat#orb2992954) with an affinity constant of 2.59 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 49.1 kDa. Observed: 70-95 kDa
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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