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Recombinant Human GCGR N-ECD Protein

Recombinant Human Glucagon receptor is produced by our Mammalian expression system and the target gene encoding Ala26-Lys136 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Glucagon receptor; GCGR; GL-R

UniProt

P47871

Expression Region

Ala26-Lys136

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Human GCGR N-ECD (C-Fc) (Cat#orb2992951) at 10 μg/ml (100 μl/well) can bind Human GCGR Antibody (Volagidemab) (Cat#orb2992815) . The ED50 of Human GCGR Antibody (Volagidemab) (Cat#orb2992815) is 65.57 ng/ml. (Regularly tested) . BLI: Loaded Human GCGR Antibody (Volagidemab) (Cat#orb2992815) on SA Biosensor, can bind Recombinant Human GCGR N-ECD (C-Fc) (Cat#orb2992951) with an affinity constant of 0.581 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 40 kDa. Observed: 47-75 kDa

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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