Recombinant Human GCGR N-ECD Protein
Recombinant Human Glucagon receptor is produced by our Mammalian expression system and the target gene encoding Ala26-Lys136 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Glucagon receptor; GCGR; GL-R
UniProt
P47871
Expression Region
Ala26-Lys136
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Recombinant Human GCGR N-ECD (C-Fc) (Cat#orb2992951) at 10 μg/ml (100 μl/well) can bind Human GCGR Antibody (Volagidemab) (Cat#orb2992815) . The ED50 of Human GCGR Antibody (Volagidemab) (Cat#orb2992815) is 65.57 ng/ml. (Regularly tested) . BLI: Loaded Human GCGR Antibody (Volagidemab) (Cat#orb2992815) on SA Biosensor, can bind Recombinant Human GCGR N-ECD (C-Fc) (Cat#orb2992951) with an affinity constant of 0.581 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 40 kDa. Observed: 47-75 kDa
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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