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Recombinant Human HLA-A*0201 GP100 complex V2 Protein

Recombinant Human HLA-A*0201 GP100 complex Proteinis produced by our Mammalian expression system and the target gene encoding (YLEPGPVTV) (Ala288Val) &Ile21-Met119&Gly25-Ile308 (Ala269Val) is expressed with a 10His tag at C-terminus.

Product Specifications

Product Name Alternative

HLA-A*0201 GP100 complex Protein

UniProt

A0A140T913, P61769

Expression Region

(YLEPGPVTV) (Ala288Val) &Ile21-Met119&Gly25-Ile308 (Ala269Val)

Conjugation

Unconjugated

Tag

C-10His

Origin

Human

Field of Research

Cell Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Anti-HLA mAb (Human IgG1) at 2μg/ml (100 μl/well) can bind Recombinant Human HLA-A*0201 GP100 complex Protein V2 (C-10His) (Cat#orb2992910) . The ED50 of Recombinant Human HLA-A*0201 GP100 complex Protein V2 (C-10His) (Cat#orb2992910) is 0.81μg/ml. (Regularly tested) .

Form

Lyophilized

Molecular Weight

Predicted: 51 KDa. Observed: 50-61 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.

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