Recombinant Human HLA-A*0201 GP100 complex V2 Protein
Recombinant Human HLA-A*0201 GP100 complex Proteinis produced by our Mammalian expression system and the target gene encoding (YLEPGPVTV) (Ala288Val) &Ile21-Met119&Gly25-Ile308 (Ala269Val) is expressed with a 10His tag at C-terminus.
Product Specifications
Product Name Alternative
HLA-A*0201 GP100 complex Protein
UniProt
A0A140T913, P61769
Expression Region
(YLEPGPVTV) (Ala288Val) &Ile21-Met119&Gly25-Ile308 (Ala269Val)
Conjugation
Unconjugated
Tag
C-10His
Origin
Human
Field of Research
Cell Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Anti-HLA mAb (Human IgG1) at 2μg/ml (100 μl/well) can bind Recombinant Human HLA-A*0201 GP100 complex Protein V2 (C-10His) (Cat#orb2992910) . The ED50 of Recombinant Human HLA-A*0201 GP100 complex Protein V2 (C-10His) (Cat#orb2992910) is 0.81μg/ml. (Regularly tested) .
Form
Lyophilized
Molecular Weight
Predicted: 51 KDa. Observed: 50-61 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.
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