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Recombinant Cynomolgus BTN3A1 Protein

Recombinant Cynomolgus Butyrophilin subfamily 3 member A1 is produced by our Mammalian expression system and the target gene encoding Gln1-Ser218 is expressed with a 6His tag at C-terminus.

Product Specifications

Product Name Alternative

Butyrophilin subfamily 3 member A1; CD277; BTN3A1; BTF5

UniProt

A0A330KXS9

Expression Region

Gln1-Ser218

Tag

C-6His

Origin

Cynomolgus

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Cynomolgus BTN3A1 (C-6His) (Cat#orb2992863) at 5 μg/ml (100 μl/well) can bind Anti-BTN3A mAb (Human IgG1) . The ED50 of Anti-BTN3A mAb (Human IgG1) is 3.0 ng/ml. (Regularly tested) . BLI: Loaded Recombinant Cynomolgus BTN3A1 (C-6His) (Cat#orb2992863) on HIS1K Biosensor, can bind Anti-BTN3A antibody (Human IgG1) with an affinity constant of 1.58 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 24.3 KDa. Observed: 24-36 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4

Available Sizes

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