Recombinant Human PCSK9 V2 Protein
Recombinant Human Proprotein convertase subtilisin/kexin type 9 is produced by our Mammalian expression system and the target gene encoding Gln31-Gln692 is expressed with a 6His tag at C-terminus.
Product Specifications
Product Name Alternative
Proprotein Convertase Subtilisin/Kexin Type 9; Neural Apoptosis-Regulated Convertase 1; NARC-1; Proprotein Convertase 9; PC9; Subtilisin/Kexin-Like Protease PC9; PCSK9; NARC1
UniProt
Q8NBP7
Expression Region
Gln31-Gln692
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Metabolism Research
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Anti-Human PCSK9 mAb (Human IgG1) at 2μg/ml (100 μl/well) can bind Recombinant Human PCSK9 V2 (C-6His) (Cat#orb2992796) . The ED50 of Recombinant Human PCSK9 V2 (C-6His) (Cat#orb2992796) is 40.84 ng/ml. (Regularly tested) . BLI: Loaded Recombinant Human PCSK9 V2 (C-6His) (Cat#orb2992796) on HIS1K Biosensor, can bind Human PCSK9 Antibody (Human IgG1) with an affinity constant of 6.17 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 71.9 KDa. Observed: (15-19) & (60-80) KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM NaH2PO4, 150mM NaCl, 0.1M Arginine, 0.1M Glu, 0.01% Tween20, pH 7.4.
Available Sizes
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