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Recombinant Human Frizzled-5 Protein

Recombinant Human Frizzled-5 is produced by our Mammalian expression system and the target gene encoding Ala27-Pro167 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Frizzled-5; FZD5; hFz5; Fz-5; Fzd5

UniProt

Q13467

Expression Region

Ala27-Pro167

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) at 5 μg/ml (100 μl/well) can bind Recombinant Human Frizzled-5 (C-Fc) (Cat#orb2992763) . The ED50 of Recombinant Human Frizzled-5 (C-Fc) (Cat#orb2992763) is 7.67 ng/ml. (Regularly tested) .. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on SA Biosensor, can bind Recombinant Human Frizzled-5 (C-Fc) (Cat#orb2992763) with an affinity constant of 17 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 42.9 KDa. Observed: 45-65 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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