Recombinant Human Frizzled-5 Protein
Recombinant Human Frizzled-5 is produced by our Mammalian expression system and the target gene encoding Ala27-Pro167 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Frizzled-5; FZD5; hFz5; Fz-5; Fzd5
UniProt
Q13467
Expression Region
Ala27-Pro167
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) at 5 μg/ml (100 μl/well) can bind Recombinant Human Frizzled-5 (C-Fc) (Cat#orb2992763) . The ED50 of Recombinant Human Frizzled-5 (C-Fc) (Cat#orb2992763) is 7.67 ng/ml. (Regularly tested) .. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on SA Biosensor, can bind Recombinant Human Frizzled-5 (C-Fc) (Cat#orb2992763) with an affinity constant of 17 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 42.9 KDa. Observed: 45-65 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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