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TfR Human Recombinant Protein (Biotin)

Recombinant Human Transferrin receptor protein 1 is produced by our Mammalian expression system and the target gene encoding Cys89-Phe760 is expressed with a 6His, Avi tag at N-terminus.

Product Specifications

Product Name Alternative

Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90

UniProt

P02786

Expression Region

Cys89-Phe760

Conjugation

Biotin

Tag

N-6His-Avi

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Human Transferrin (C-6His) (Cat#orb2994488) at 5μg/ml (100 μl/well) can bind Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) . The ED50 of Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) is 22.33 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human TFRC mAb (Human IgG1) on Pro-A Biosensor, can bind Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) with an affinity constant of 1.69 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 78.1 KDa. Observed: 80-95 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween80, pH 7.4.

Available Sizes

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