Recombinant Mouse Frizzled-10 Protein
Recombinant Mouse Frizzled-10 is produced by our Mammalian expression system and the target gene encoding Ile22-Gly162 is expressed with a 6His tag at C-terminus.
Product Specifications
Product Name Alternative
Frizzled-10; Fz-10; Fzd10; Fz10; FZD10
UniProt
Q8BKG4
Expression Region
Ile22-Gly162
Tag
C-6His
Origin
Mouse
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Recombinant Human Frizzled-10 (C-6His) (Cat#orb2992707) at 2 μg/ml (100 μl/well) can bind Anti-Human Frizzled-10 mab (Human IgG1) . The ED50 of Anti-Human Frizzled-10 mab (Human IgG1) is 6.786 ng/ml. (Regularly tested) . BLI: Loaded Recombinant Mouse Frizzled-10 (C-6His) (Cat#orb2992707) on HIS1K Biosensor, can bind Anti-Human Frizzled-10 mab (Human IgG1) with an affinity constant of 23.1 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 16.8 KDa. Observed: 20-27 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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