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Recombinant Human CALCRL&RAMP1 Protein

Biotinylated Recombinant Human CALCRL&RAMP1 Heterodimer Protein is produced by our Mammalian expression system and the target gene encoding Glu23-Ile146&Cys27-Ser117 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Product Specifications

Product Name Alternative

CALCRL&RAMP1 Heterodimer Protein

UniProt

O60894, Q16602

Expression Region

Glu23-Ile146&Cys27-Ser117

Tag

C-Fc-Avi

Origin

Human

Field of Research

Signal Transduction

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Human CGRP Antibody (Erenumab, Research Grade) (Cat#orb2992589) at 2 μg/ml (100 μl/well) can bind Biotinylated Human CALCRL&RAMP1 Heterodimer Protein (C-Fc-Avi) (Cat#orb2992606) . (Regularly tested) . BLI: Loaded Biotinylated Human CALCRL&RAMP1 Heterodimer Protein (Cat#orb2992606) on SA Biosensor, can bind Human CGRP Antibody (Erenumab, Research Grade) (Cat#orb2992589) with an affinity constant of 2.08 pM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 42.9&39 KDa. Observed: (40-44) & (50-65) KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.4

Available Sizes

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