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Recombinant Human Frizzled-7 Protein

Recombinant Human Frizzled-7 is produced by our Mammalian expression system and the target gene encoding Gln33-Leu185 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Frizzled-7; FZD7

UniProt

O75084

Expression Region

Gln33-Leu185

Tag

C-6His

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Human Frizzled-7 N-ECD (C-6His) (Cat#orb2992571) at 5 μg/ml (100 μl/well) can bind Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) . The ED50 of Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) is 10.57 ng/ml. (Regularly tested) .. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on Pro-A Biosensor, can bind Recombinant Human Frizzled-7 N-ECD (Cat#orb2992571) with an affinity constant of 0.346 uM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 17.5 KDa. Observed: 23-35 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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