Recombinant Human C5AR1 N-ECD Protein
Recombinant HumanC5a anaphylatoxin chemotactic receptor 1 is produced by our Mammalian expression system and the target gene encoding Met1-Asp37 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
C5a anaphylatoxin chemotactic receptor 1; C5AR1; C5a-R; C5aR; CD88; C5AR; C5R1
UniProt
P21730
Expression Region
Met1-Asp37
Tag
C-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) . SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
ELISA: Immobilized Human C5AR1 Antibody (Avdoralimab) at 10 μg/ml (100 μl/well) can bind Recombinant Human C5AR1 N-ECD (C-Fc) (Cat#orb2992554) *.*: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human C5AR1 N-ECD (C-Fc) (Cat#orb2992554) is 2.623 ug/ml. (Regularly tested) .
Form
Lyophilized
Molecular Weight
Predicted: 31.2 KDa. Observed: 38-50 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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