Recombinant Macaca mulatta Coagulation factor XI (F11) (Active)
This Recombinant Macaca mulatta Coagulation factor XI (TrEMBL) spans the amino acid sequence from region 19-624aa. Purity: Greater than 95% as determined by SDS-PAGE.
Product Specifications
Product Name Alternative
Coagulation factor XI; EGK_16300
UniProt
G7MSF8
Expression Region
19-624aa
Tag
C-terminal 10xHis-tagged
Source
Mammalian cell
Origin
Macaca mulatta (Rhesus macaque)
Field of Research
Cardiovascular Research
Purity
Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Bioactivity
1Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 at 2 μg/mL can bind Anti-F11 recombinant antibody. The EC50 is 0.5654-0.6444 ng/mL. 2Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC) . The specific activity is >150 pmol/min/μg.
Form
Lyophilized powder
Molecular Weight
69.3 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
ECVTRLFKDIHFEGGDIATVFTPSAKHCQVVCTHHPRCLLFTFTGESASEDPTQWFTCVLKDSVTETLPRVNRTGAISGYSFKQCSHQISACNKDIYVDLDMKGINYNSSLATSAQECQERCTDDVHCHFFTYATRQFPSLEHRNICLLKHTQTGTPTGIMKLDKVVTGFSLKSCALSNLACIRDVFPNTVFADSNIDSVMAPDAFVCRRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPRTRIKKSKALSGFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAVKGHEACQKLCTNAVRCQFFTYAPAQASCNEGKGKCYLKLSSNGSPTKILRGTGGISGYTLRLCKMDNECTTKIKPRIVGGTASVRGEWPWQVTLHTTSPTQRHLCGGSIIGNQWILTAAHCFYGVESPKILRVYIGILNQSEIKEDTSFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYTDSQRPICLPSKGDRNVIYTDCWVTGWGYRKLRDKIQNTLQKAKIPLVTNDECQKRYRGHKITHKMICAGYREGGKDACKGDSGGPLSCKHNEVWHLVGITSWGEGCAQRERPGVYTNVLEYVDWILEKTQA
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