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Recombinant Human Inhibin beta A chain (INHBA) (Active)

This Recombinant Human Inhibin beta A chain (INHBA) (Active) spans the amino acid sequence from region 21-426aa. Purity: Greater than 95% as determined by SDS-PAGE.

Product Specifications

Product Name Alternative

Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein (EDF) ; INHBA

UniProt

P08476

Expression Region

21-426aa

Tag

N-terminal 10xHis-tagged

Source

Mammalian cell

Origin

Homo sapiens (Human)

Field of Research

Cardiovascular Research

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Bioactivity

1Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 0.9519-1.213 ng/mL. 2Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Human ACVR2A. The EC50 is 74.75-84.73 ng/mL. 3Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Human ACVR2B. The EC50 is 45.93-52.42 ng/mL. 4Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Anti-INHBA recombinant antibody. The EC50 is 10.28-12.68 ng/mL.

Form

Lyophilized powder

Molecular Weight

46.5 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

AA Sequence

SPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVKKHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELMEQTSEIITFAESGTARKTLHFEISKEGSDLSVVERAEVWLFLKVPKANRTRTKVTIRLFQQQKHPQGSLDTGEEAEEVGLKGERSELLLSEKVVDARKSTWHVFPVSSSIQRLLDQGKSSLDVRIACEQCQESGASLVLLGKKKKKEEEGEGKKKGGGEGGAGADEEKEQSHRPFLMLQARQSEDHPHRRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Available Sizes

Frequently Asked Questions

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