Recombinant Human Inhibin beta A chain (INHBA) (Active)
This Recombinant Human Inhibin beta A chain (INHBA) (Active) spans the amino acid sequence from region 21-426aa. Purity: Greater than 95% as determined by SDS-PAGE.
Product Specifications
Product Name Alternative
Inhibin beta A chain; Activin beta-A chain; Erythroid differentiation protein (EDF) ; INHBA
UniProt
P08476
Expression Region
21-426aa
Tag
N-terminal 10xHis-tagged
Source
Mammalian cell
Origin
Homo sapiens (Human)
Field of Research
Cardiovascular Research
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
1Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 0.9519-1.213 ng/mL. 2Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Human ACVR2A. The EC50 is 74.75-84.73 ng/mL. 3Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Human ACVR2B. The EC50 is 45.93-52.42 ng/mL. 4Measured by its binding ability in a functional ELISA.Immobilized Human INHBA at 2 μg/mL can bind Anti-INHBA recombinant antibody. The EC50 is 10.28-12.68 ng/mL.
Form
Lyophilized powder
Molecular Weight
46.5 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
AA Sequence
SPTPGSEGHSAAPDCPSCALAALPKDVPNSQPEMVEAVKKHILNMLHLKKRPDVTQPVPKAALLNAIRKLHVGKVGENGYVEIEDDIGRRAEMNELMEQTSEIITFAESGTARKTLHFEISKEGSDLSVVERAEVWLFLKVPKANRTRTKVTIRLFQQQKHPQGSLDTGEEAEEVGLKGERSELLLSEKVVDARKSTWHVFPVSSSIQRLLDQGKSSLDVRIACEQCQESGASLVLLGKKKKKEEEGEGKKKGGGEGGAGADEEKEQSHRPFLMLQARQSEDHPHRRRRRGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
Available Sizes
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