HDAC6 Antibody

Goat polyclonal antibody to HDAC6, which is a member of the histone deacetylase family, HD type 2 subfamily. It is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. It acts in microtubule-dependent cell motility via deacetylation of tubulin. HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network.

Product Specifications

Product Name Alternative

Anti HDAC6 antibody, anti histone deacetylase 6 antibody, anti HD6 antibody, anti JM21 antibody, anti KIAA0901 antibody

Reactivity

Human

Target

HDAC6

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

AHQNKFGEDMPHPH

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, IHC-P: 5 μg/ml

Molecular Weight

131; 132.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 150kDa band observed in nuclear lysates of cell lines NIH3T3, and HeLa (calculated MW of 131kDa according to NP_006035.2) . Recommended concentration: 1-3 μg/ml.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, FC, IF, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.