Recombinant Macaca fascicularis Interleukin-2 (IL2) (Active)
This Recombinant Macaca fascicularis Interleukin-2 (IL2) spans the amino acid sequence from region 21-154aa. Purity: Greater than 90% as determined by SDS-PAGE.
Product Specifications
Product Name Alternative
Interleukin-2; IL-2; T-cell growth factor; TCGF
UniProt
Q29615
Expression Region
21-154aa
Tag
C-terminal hFc1-tagged
Source
Mammalian cell
Origin
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Field of Research
Immunology & Inflammation
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 90% as determined by SDS-PAGE.
Bioactivity
1Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Macaca fascicularis IL2. The EC50 is 88.60-116.8 ng/mL. 2Macaca fascicularis IL2 captured on Protein A Chip can bind Recombinant Macaca fascicularis IL2RA with an affinity constant of 31.3 nM as detected by MetaSPR Assay.
Form
Lyophilized powder
Molecular Weight
44.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELRHLQCLEEELKPLEEVLNLAQSKSFHLRDTKDLISNINVIVLELKGSETTLMCEYADETATIVEFLNRWITFCQSIISTLT
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