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Goat betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody

Goat polyclonal to beta CstF-64 variant 1.

Product Specifications

Product Name Alternative

Anti 64kDa antibody, anti alphaCstF-64 variant 4 antibody, anti betaCstF-64 variant 1 antibody, anti betaCstF-64 variant 3 antibody, anti C630034J23Rik antibody, anti CF-1 64 kDa subunit antibody, anti cleavage stimulation factor 64 kDa subunit antibody, anti cleavage stimulation factor subunit 2 antibody, anti cleavage stimulation factor, 3' pre-RNA subunit 2 antibody, anti cleavage stimulation factor, 3' pre-RNA subunit 2, 64 kDa antibody, anti CSTF 64 kDa subunit antibody, anti Cstf2 antibody, anti Cstf64 antibody, anti cstF-64 antibody

Reactivity

Human, Mouse

Target

BetaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

PASIERVQGQRT

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, IHC-P: 5 μg/ml

Molecular Weight

61.3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: In transfected HeLa transiently expressing Mouse BCstf-64 ((DYKDDDDK tagged), a band of approx. 80 kDa was observed using anti-DYKDDDDK antibody in Western blot after immunoprecipitation. No bands were observed in mock-transfected HeLa. Yields were similar between RIPA buffer and Triton X-100 buffer conditions. Recommended amount, 2 ug per lysate.

Tested Applications

ELISA, IHC, IP

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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