Recombinant Human IL-4 Protein
Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed.
Product Specifications
Product Name Alternative
Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
UniProt
P05112
Expression Region
His25-Ser153
Tag
Tag free
Field of Research
Immunology & Inflammation
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Cynomolgus IL-4RA-His (Cat#orb2994860) on HIS1K Biosensor, can bind Human IL-4 (Cat#orb3002477) with an affinity constant of 0.33 nM as determined in BLI assay. Loaded Cynomolgus IL-4RA-Fc (Cat#orb2993918) on Protein A Biosensor, can bind Human IL-4 (Cat#orb3002477) with an affinity constant of 0.54 nM as determined in BLI assay. Loaded Human IL-4RA-His (Cat#orb2994200) on HIS1K Biosensor, can bind Human IL-4 (Cat#orb3002477) with an affinity constant of 0.48 nM as determined in BLI assay. Loaded Human IL-4RA-Fc (Cat#orb2994196) on Protein A Biosensor, can bind Human IL-4 (Cat#orb3002477) with an affinity constant of 0.99 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using TF‐1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.05 ng/ml. (QC verified)
Form
Lyophilized
Molecular Weight
Predicted: 14.97 KDa. Observed: 18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Available Sizes
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