Recombinant Human TF Protein
Recombinant Human Coagulation Factor III/Tissue Factor is produced by our Mammalian expression system and the target gene encoding Gly34-Glu251 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142; F3
UniProt
P13726
Expression Region
Gly34-Glu251
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human TF (C-6His) (Cat#orb2995049) at 1μg/ml (100 μl/well) can bind Anti-Human TF Antibody (TIS_bio, Research Grade) (Cat#orb2992713) .The EC50 of Anti-Human TF Antibody (TIS_bio, Research Grade) (Cat#orb2992713) is ng/ml. (Regularly tested) .. BLI: Loaded Anti-TF-mAb on Pro-A Biosensor, can bind Recombinant Human TF (C-6His) (Cat#orb2995049) with an affinity constant of 9.69 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 25.76 KDa. Observed: 35-55 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Available Sizes
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