Recombinant Human PD-1 Protein

Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Programmed cell death protein 1; hPD-1; PDCD1; CD279

UniProt

Q15116

Expression Region

Pro21-Gln167

Conjugation

Unconjugated

Tag

C-mFc

Origin

Human

Field of Research

Cancer Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)

Bioactivity

ELISA: Immobilized Anti-Human PD-1 mAb (Cat#GMP-A085) at 2μg/ml (100 μl/well) can bind Human PD-1-mFc (Cat#orb2994924) . The ED50 of Human PD-1-mFc (Cat#orb2994924) is 12.53 ng/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc (Cat#GMP-A085) on AHC Biosensor, can bind Human PD-1-His (Cat#orb2994924) with an affinity constant of -3 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 42 KDa. Observed: 60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.