Recombinant Human 5T4 Protein (Biotin)
Biotinylated Recombinant Human Trophoblast Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a Avi, 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
TPBG; 5T4; 5T4AG; M6P1; WAIF1
UniProt
Q13641
Expression Region
Ser32-Ser355
Conjugation
Biotin
Tag
C-Avi-6His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human TPBG Antibody (5G9) (Human IgG1) (Cat#orb2992640) at 2 μg/ml (100 μl/well) can bind Biotinylated Human TPBG-Avi-His (Cat#orb2993903) . The ED50 of Human Biotinylated TPBG-Avi-His (Cat#orb2993903) is 0.21 ng/ml. (QC verified) .. BLI: LoadedHuman TPBG Antibody (5G9) (Human IgG1) (Cat#orb2992640) on Protein-A Biosensor, can bind Biotinylated Human 5T4 (C-Avi-6His) (Cat#orb2993903) with an affinity constant of 9.29 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 38 KDa. Observed: 50-90 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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