Recombinant Human IL-6RA Protein
Recombinant Human Interleukin-6 receptor subunit alpha is produced by our Mammalian expression system and the target gene encoding Leu20-Pro365 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6R 1; Membrane glycoprotein 80; gp80; CD126
UniProt
P08887
Expression Region
Leu20-Pro365
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human IL6R Antibody (SAR_bio, Research Grade) (Cat#NC373) at 1μg/ml (100 μl/well) can bind Recombinant Human IL -6RA (C-Fc) (Cat#orb2993733) *. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human IL -6RA (C-Fc) (Cat#orb2993733) is 1.78 ng/ml. (Regularly tested) . BLI: Loaded Recombinant Rhesus Macaque IL-6 (C-6His) (Cat#orb2992743) on HIS1K Biosensor, can bind Recombinant Human IL-6RA (C-Fc) (Cat#orb2993733) with an affinity constant of 21.6 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 65.4 KDa. Observed: 85-100 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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