Recombinant Human CD40L Protein

Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Glu108-Leu261 is expressed with a 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

UniProt

P29965

Expression Region

Glu108-Leu261

Conjugation

Unconjugated

Tag

N-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Human CD40-Fc (Cat#orb2994010) on Protein A Biosensor, can bind Human CD40L-His (Cat#orb2994033) with an affinity constant of 2.74 nM as determined in BLI assay. Loaded Anti-Human CD40L mAb-Fc (Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L-His (Cat#orb2994033) with an affinity constant of -3 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 19.1 KDa. Observed: 19-22 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.