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Recombinant Human FcRn Heterodimer Protein

Recombinant Human IgG Fc Fragment Receptor Transporter is produced by our Mammalian expression system and the target gene encoding Ala24-Ser297&Ile21-Met119 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

IgG receptor FcRn; Neonatal Fc receptor; FCRN

UniProt

P61769

Expression Region

Ala24-Ser297&Ile21-Met119

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Endotoxin

<0.1EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)

Bioactivity

BLI: Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Human IgG1) (Cat#GMP-A062) with an affinity constant of 11.3 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Anti-Human CD137 mAb (Human IgG4) (Cat#orb2993114) with an affinity constant of 0.376 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG1 Fc (Cat#orb2993960) with an affinity constant of 98.6 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG1 Fc (Cat#orb2992939) with an affinity constant of 0.265 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG2 Fc (223AA) (Cat#orb2993943) with an affinity constant of 85.7 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG2 Fc (228AA) (Cat#orb2993938) with an affinity constant of 74.5 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG3 Fc (Cat#orb2993894) with an affinity constant of 0.101 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG4 Fc (228AA) (Cat#orb2993937) with an affinity constant of 0.127 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human FcRn Heterodimer (C-6His) (Cat#orb2994565) on HIS1K Biosensor, can bind Recombinant Human IgG4 Fc (224AA) (Cat#orb2993942) with an affinity constant of 48 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 31.2&11.7 KDa. Observed: 37&13 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4.

Available Sizes

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