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Recombinant Human TIGIT Protein

Recombinant Human T-cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met22-Pro141 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3; Tigit; VSIG9; VSTM3

UniProt

Q495A1

Expression Region

Met22-Pro141

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Recombinant Mouse PVR (C-6His) (Cat#orb2992802) on HIS1K Biosensor, can bind Recombinant Human TIGIT (C-Fc) (Cat#orb2994151) with an affinity constant of 17.6 nM as determined in BLI assay. (Regularly tested) Loaded Human PVR-His (Cat#orb2993514) on HIS1K Biosensor, can bind Human TIGIT-Fc (Cat#orb2994151) with an affinity constant of 8.58 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 39.7 KDa. Observed: 40-55 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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