Recombinant Human BAFF Protein (Biotin)
Biotinylated Recombinant Human B-cell-activating factor is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a Avi tag and a Fc tag at the N-terminus.
Product Specifications
Product Name Alternative
Tumor necrosis factor ligand superfamily member 13B; TNFSF13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257; TNFSF20; ZTNF4
UniProt
Q9Y275
Expression Region
Ala134-Leu285
Conjugation
Biotin
Tag
N-Avi-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human BCMA-His-Flag (Cat#orb2993882) at 5μg/ml (100 μl/well) can bind Biotinylated Human BAFF-Avi-Fc (Cat#orb2993660) . The ED50 of Biotinylated Human BAFF-Avi-Fc (Cat#orb2993660) is 29.46 ng/ml. (Regularly tested) Immobilized Recombinant Human BCMA-His-Flag (Cat#orb2993882) at 5μg/ml (100 μl/well) can bind Biotinylated Human BAFF-Avi-Fc (Cat#orb2993660) . The ED50 of Biotinylated Human BAFF-Avi-Fc (Cat#orb2993660) is 29.46 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 44.8 KDa. Observed: 50-60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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