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Recombinant Human Siglec-3 Protein

Recombinant Human Myeloid cell surface antigen CD33 is produced by our Mammalian expression system and the target gene encoding Asp18-His259 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Myeloid Cell Surface Antigen CD33; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67; CD33; SIGLEC3

UniProt

P20138

Expression Region

Asp18-His259

Tag

C-Fc

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Human Siglec-3 (C-Fc) (Cat#orb2992883) at 2μg/ml (100 μl/well) can bind Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) *. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) is 7.465 ng/ml. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 53.9 KDa. Observed: 65-95 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

Frequently Asked Questions

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