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Recombinant Human CALCRL&RAMP1 Heterodimer (C-Fc) Protein

Recombinant Human CALCRL&RAMP1 Heterodimer Protein is produced by our Mammalian expression system and the target gene encoding Glu23-Ile146&Cys27-Ser117 is expressed with a human IgG1 Fc hole and human IgG1 Fc knob tag at the C-terminus respectively.

Product Specifications

Product Name Alternative

CALCRL&RAMP1 complex Protein

UniProt

O60894, Q16602

Expression Region

Glu23-Ile146&Cys27-Ser117

Conjugation

Unconjugated

Tag

C-Fc&C-Fc

Origin

Human

Field of Research

Signal Transduction

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Human CALCRL&RAMP1 Heterodimer Protein (C-Fc) (Cat#orb2992774) at 2μg/ml (100 μl/well) can bind Anti-Human CALCRL&RAMP1 mAb (Erenumab) *. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CALCRL&RAMP1 mAb (Erenumab) is 16.13 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human CALCRL&RAMP1 mAb (Human IgG2) on SA Biosensor, can bind Recombinant Human CALCRL&RAMP1 Heterodimer Protein (C-Fc) (Cat#orb2992774) with an affinity constant of 0.987 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 41.1&37.2 KDa. Observed: (36-43) & (46-66) KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4.

Available Sizes

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