Recombinant Human Laminin 511 Protein
Recombinant Human Laminin511 is produced by our Mammalian expression system and the target gene encoding Ala2534-Ala3323&Leu1561-Leu1786&Asn1364-Pro1609 is expressed with a His, Flag and HA tag at N terminus respectively.
Product Specifications
Product Name Alternative
Laminin 511; LN511
UniProt
P11047, P07942, O15230
Expression Region
Ala2534-Ala3323&Leu1561-Leu1786&Asn1364-Pro1609
Tag
N-His & N-Flag & N-HA
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 4 Heterodimer (C-Flag, C-6His) (Cat#orb2992902) at 10μg/ml (100 μl/well) can bind Recombinant Human Laminin511 E8 Protein (Cat#orb3002508) .The ED50 of Recombinant Human Laminin511 E8 Protein (Cat#orb3002508) is 40.21 ng/ml (Regularly tested) .. Cell Based Assay: Measured by the ability of the immobilized protein to support the adhesion of H9 Human embryonic stem cells. The ED50 for this effect is 0.02017 μg/mL
Form
Lyophilized
Molecular Weight
Predicted: 86.7&26.4&28.5 KDa. Observed: (32-36) & (36-49) & (100-120) KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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