Recombinant Human Frizzled-8 Protein
Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
UniProt
Q9H461
Expression Region
Ala28-Pro172
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human Frizzled-8 (C-6His) (Cat#orb2993788) at 2μg/ml (100μl/well) can bind Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) . The ED50 of Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) is 10.74 ng/ml. (Regularly tested) .. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on Pro-A Biosensor, can bind Recombinant Human Frizzled-8 (C-6His) (Cat#orb2993788) with an affinity constant of 4.24 μM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 17.3 KDa. Observed: 22-32 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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