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14-3-3 sigma/Stratifin, Biotinylated Antibody

Goat polyclonal antibody against SFN conjugated to Biotin.

Product Specifications

Product Name Alternative

SFN; stratifin; YWHAS; 14-3-3 sigma; epithelial cell marker protein 1

Reactivity

Human, Mouse

Target

14-3-3 sigma / Stratifin, Biotinylated

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CDLHTLSEDSYKDST.

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.1-0.3 μg/ml

Molecular Weight

27.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 28kDa band observed in Mouse Skin lysates (calculated MW of 27.7kDa according to Mouse NP_061224.2) . See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3

Prediction Reactivity

Canine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

2810

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