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SOX2 Goat Polyclonal Antibody

SOX2 Goat Polyclonal Antibody recognizes the N-terminus of human SOX2. Validated for ELISA (1:32,000), WB (0.1-0.3 μg/ml), IHC (6-8 μg/ml), and flow cytometry (10 μg/ml) . Reactivity includes human, with predicted reactivity for bovine, canine, and rat. For research use only.

Product Specifications

Product Name Alternative

SOX2; SRY (sex determining region Y) -box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2

UniProt

P48431

Reactivity

Human

Immunogen

Peptide with the sequence YNMMETELKPPGPQQ-C, derived from the N-terminus of the protein sequence corresponding to NP_003097.1.

Target

SOX2

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

P-ELISA: 1: 32000, WB: 0.1-0.3μg/ml, IHC: 6-8μg/ml, FC: 10ug/ml

Molecular Weight

34.3 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 40kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3kDa according to) NP_003097.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3μg/m. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8μg/ml.. Notes: Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Prediction Reactivity

Bovine, Canine, Rat

Tested Applications

ELISA, FC, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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