Doublecortex/DCX Antibody
Goat polyclonal antibody to DCX
Product Specifications
Product Name Alternative
DCX ; doublecortex; lissencephaly; X-linked (doublecortin) ; RP5-914P14.1; DBCN; DC; LISX; SCLH; XLIS ; OTTHUMP00000062892; doublecortin; doublin; lissencephalin-X
Reactivity
Human, Mouse
Target
Doublecortex / DCX
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KASPTPQKTSAKSPG
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.003-0.01 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
49.3; 40.5; 40.0
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to Mouse NP_001103692.1) . Recommended concentration: 0.003-0.01μg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Brain (Cortex) . Recommended concentration: 7μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5μg/ml.
Prediction Reactivity
Canine, Feline, Rat
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1641
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