Recombinant Human C-C chemokine receptor type 6 (CCR6) -VLPs (Active)

Product Specifications

Product Name Alternative

CKRL3; CMKBR6; GPR29; STRL22

Abbreviation

Recombinant Human CCR6 protein-VLPs (Active)

Gene Name

CCR6

UniProt

P51684

Expression Region

1-374aa

Organism

Homo sapiens (Human)

Target Sequence

MSGESMNFSDVFDSSEDYFVSVNTSYYSVDSEMLLCSLQEVRQFSRLFVPIAYSLICVFGLLGNILVVITFAFYKKARSMTDVYLLNMAIADILFVLTLPFWAVSHATGAWVFSNATCKLLKGIYAINFNCGMLLLTCISMDRYIAIVQATKSFRLRSRTLPRSKIICLVVWGLSVIISSSTFVFNQKYNTQGSDVCEPKYQTVSEPIRWKLLMLGLELLFGFFIPLMFMIFCYTFIVKTLVQAQNSKRHKAIRVIIAVVLVFLACQIPHNMVLLVTAANLGKMNRSCQSEKLIGYTKTVTEVLAFLHCCLNPVLYAFIGQKFRNYFLKILKDLWCVRRKYKSSGFSCAGRYSENISRQTSETADNDNASSFTM

Tag

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Type

MP-VLP Transmembrane Protein & Active Protein & In Stock Protein

Source

Mammalian cell

Field of Research

Cancer

Relevance

Act as a receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Act as a receptor for non-chemokine ligands such as beta-defensins.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Purity

The purity information is not available for VLPs proteins.

Activity

Yes

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CCR6 at 10 μg/mL can bind Anti-CCR6 recombinant antibody (CSB-RA004845MA1HU) . The EC50 is 44.79-56.10 ng/mL. The VLPs (CSB-MP3838) is negative control.

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Molecular Weight

42.5 kDa

References & Citations

"Identification of CCR6, the specific receptor for a novel lymphocyte- directed CC chemokine LARC." Baba M., Imai T., Nishimura M., Kakizaki M., Takagi S., Hieshima K., Nomiyama H., Yoshie O. J. Biol. Chem. 272:14893-14898 (1997) "Molecular cloning and RNA expression of two new human chemokine receptor- like genes." Zaballos A., Varona R., Gutierrez J., Lind P., Marquez G. Biochem. Biophys. Res. Commun. 227:846-853 (1996) "Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27." Liao F., Lee H.-H., Farber J.M. Genomics 40:175-180 (1997)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.