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AKT1 Antibody

Goat polyclonal antibody to AKT1

Product Specifications

Product Name Alternative

AKT1, v-akt murine thymoma viral oncogene homolog 1 , HGNC:391, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA , RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha

Reactivity

Human, Mouse

Target

AKT1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EVSLAKPKHR

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 2-3ug/ml

Molecular Weight

55.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 60kDa band observed in MCF7 and approx. 60-65kDa in NIH3T3 cell lysates (calculated MW of 55.7kDa according to Human NP_005154.2 and Mouse NP_033782.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 2-3ug

Prediction Reactivity

Bovine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

207

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