Lipopolysaccharides, from E. coli O55:B5

Lipopolysaccharides, from E. coli O55:B5 (LPS, from Escherichia coli (O55:B5) ) are endotoxins and TLR4 activators extracted from Escherichia coli (E. coli O55:B5) and are classified as S (smooth) type LPS. Lipopolysaccharides, from E. coli O55:B5 possess the typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activate TLR-4 in immune cells, exhibit high pyrogenicity, and demonstrate dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 can be widely used to induce cellular inflammation and establish animal models related to inflammation[1][2][3][4][5][6][7]. It is recommended to prepare a solution with concentration ≥2 mg/mL. Vortex thoroughly for more than 10 minutes. Due to the adsorption characteristics of LPS, silanized container or low adsorption centrifuge tubes should be used for aliquoting and storage, and mix thoroughly before use.

Product Specifications

Product Name Alternative

LPS

UNSPSC

12352200

Target

Toll-like Receptor (TLR)

Type

Biochemical Assay Reagents

Related Pathways

Immunology/Inflammation

Applications

COVID-19-immunoregulation

Field of Research

Cancer; Infection

Assay Protocol

https://www.medchemexpress.com/lipopolysaccharide.html

Solubility

DMSO : 50 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 5 mg/mL (ultrasonic)

Smiles

[Lipopolysaccharides,fromE.coliO55:B5]

References & Citations

[1]Krukenberg KA, et al. Extracellular poly (ADP-ribose) is a pro-inflammatory signal for macrophages. Chem Biol. 2015 Apr 23;22 (4) :446-452.|[2]Gayle DA, et al. Lipopolysaccharide (LPS) -induced dopamine cell loss in culture: roles of tumor necrosis factor-alpha, interleukin-1beta, and nitric oxide. Brain Res Dev Brain Res. 2002 Jan 31;133 (1) :27-35.|[3]Xiao Z, et al. Ferrostatin-1 alleviates lipopolysaccharide-induced cardiac dysfunction. Bioengineered. 2021 Dec;12 (2) :9367-9376.|[4]Huang S, et al. Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety. Immunity. 2024 Mar 12;57 (3) :495-512.e11.|[5]Guo D, et al. Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform. Bioact Mater. 2024 Jul 23;41:221-238.|[6]Yang J, et al. MicroRNA-106a Provides Negative Feedback Regulation in Lipopolysaccharide-Induced Inflammation by targeting TLR4. Int J Biol Sci. 2019 Aug 22;15 (11) :2308-2319.|[7]Makrecka-Kuka M, et al. Inhibition of CPT2 exacerbates cardiac dysfunction and inflammation in experimental endotoxaemia. J Cell Mol Med. 2020 Oct;24 (20) :11903-11911.