NCKAP1 Antibody
Goat polyclonal antibody to NCKAP1
Product Specifications
Product Name Alternative
Anti FLJ11291 antibody, anti HEM2 antibody, anti KIAA0587 antibody, anti membrane-associated protein HEM-2 antibody, anti MGC8981 antibody, anti NAP 1 antibody, anti NAP1 antibody, anti NAP125 antibody, anti NCKAP1 antibody, anti NCK-associated protein 1 antibody, anti OTTHUMP00000163460 antibody, anti OTTHUMP00000163461 antibody, anti p125Nap1 antibody
Reactivity
Human
Target
NCKAP1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
TPREYLTSHLEIR
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
ELISA: 1:128000, WB: 0.1 μg/ml
Molecular Weight
129; 130
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5μg/ml antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calculated MWt.is 12. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, FC, IF
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10787
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